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      1. Author :
        Yang, Li; Johansson, Jan; Ridsdale, Ross; Willander, Hanna; Fitzen, Michael; Akinbi, Henry T; Weaver, Timothy E
      2. Title :
      3. Type :
        Journal Article
      4. Year :
      5. Publication :
        Journal of immunology (Baltimore, Md.: 1950)
      6. Products :
      7. Volume :
      8. Issue :
      9. Page Numbers :
      10. Research Area :
      11. Keywords :
        Animals; Anti-Bacterial Agents; Bronchoalveolar Lavage Fluid; Hydrogen-Ion Concentration; Immunity, Innate; Klebsiella pneumoniae; Macrophages, Alveolar; Mice; Mice, Transgenic; Protein Precursors; Protein Structure, Tertiary; Proteolipids; Saposins; Staphylococcus aureus; Tissue Distribution; Xen5
      12. Abstract :
        Surfactant protein B (SP-B) proprotein contains three saposin-like protein (SAPLIP) domains: a SAPLIP domain corresponding to the mature SP-B peptide is essential for lung function and postnatal survival; the function of SAPLIP domains in the N-terminal (SP-BN) and C-terminal regions of the proprotein is not known. In the current study, SP-BN was detected in the supernatant of mouse bronchoalveolar lavage fluid (BALF) and in nonciliated bronchiolar cells, alveolar type II epithelial cells, and alveolar macrophages. rSP-BN indirectly promoted the uptake of bacteria by macrophage cell lines and directly killed bacteria at acidic pH, consistent with a lysosomal, antimicrobial function. Native SP-BN isolated from BALF also killed bacteria but only at acidic pH; the bactericidal activity of BALF at acidic pH was completely blocked by SP-BN Ab. Transgenic mice overexpressing SP-BN and mature SP-B peptide had significantly decreased bacterial burden and increased survival following intranasal inoculation with bacteria. These findings support the hypothesis that SP-BN contributes to innate host defense of the lung by supplementing the nonoxidant antimicrobial defenses of alveolar macrophages.
      13. URL :
      14. Call Number :
        PKI @ catherine.lautenschlager @
      15. Serial :
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