1. Resources
  2. Citations Library

Citation Details

You are viewing citation details. You can save or export citation(s) below, access an article, or start a new search.

1–1 of 1 record found matching your query:

Headers act as filters

      1. Author :
        Adachi, T; Kawakami, E; Ishimaru, N; Ochiya, T; Hayashi, Y; Ohuchi, H; Tanihara, M; Tanaka, E; Noji, S
      2. Title :
        Delivery of small interfering RNA with a synthetic collagen poly (Pro Hyp Gly) for gene silencing in vitro and in vivo
      3. Type :
        Journal Article
      4. Year :
        2010
      5. Publication :
        Development, Growth & Differentiation
      6. Products :
      7. Volume :
        52
      8. Issue :
        N/A
      9. Page Numbers :
        693
      10. Research Area :
        N/A
      11. Keywords :
        IVIS, Xenogen
      12. Abstract :
        Silencing gene expression by small interfering RNAs (siRNAs) has become a powerful tool for the genetic analysis of many animals. However, the rapid degradation of siRNA and the limited duration of its action in vivo have called for an efficient delivery technology. Here, we describe that siRNA complexed with a synthetic collagen poly(Pro-Hyp-Gly) (SYCOL) is resistant to nucleases and is efficiently transferred into cells in vitro and in vivo, thereby allowing long-term gene silencing in vivo. We found that the SYCOL-mediated local application of siRNA targeting myostatin, coding a negative regulator of skeletal muscle growth, in mouse skeletal muscles, caused a marked increase in the muscle mass within a few weeks after application. Furthermore, in vivo administration of an anti-luciferase siRNA/SYCOL complex partially reduced luciferase expression in xenografted tumors in vivo. These results indicate a SYCOL-based non-viral delivery method could be a reliable simple approach to knockdown gene expression by RNAi in vivo as well as in vitro.
      13. URL :
        N/A
      14. Call Number :
        135745
      15. Serial :
        5847