1. Resources
  2. Citations Library

Citation Details

You are viewing citation details. You can save or export citation(s) below, access an article, or start a new search.

1–1 of 1 record found matching your query:

Headers act as filters

      1. Author :
        Luo, Jian; Wyss-Coray, Tony
      2. Title :
      3. Type :
        Journal Article
      4. Year :
        2009
      5. Publication :
        Bioluminescence
      6. Products :
      7. Volume :
        574
      8. Issue :
        N/A
      9. Page Numbers :
        193
      10. Research Area :
        N/A
      11. Keywords :
        TGF-?, Smad, bioluminescence imaging, in vivo, luciferase, immunofluorescence IVIS, Xenogen
      12. Abstract :
        TGF-beta signaling via the Smad2/3 pathway has key roles in development and tissue homeostasis. Perturbations of the TGF-beta signaling are involved in the pathogenesis of many human diseases, including cancer, fibrotic disorders, developmental defects, and neurodegeneration. To study the temporal and spatial patterns of Smad2/3-dependent signaling in living animals, we engineered transgenic mice with a Smad-responsive luciferase reporter (SBE-luc mice). Smad2/3-dependent signaling can be assessed non-invasively in living mice by bioluminescence imaging. To identify the cellular source of the bioluminescence signal, we generated new reporter mice expressing a trifusion protein containing luciferase, red fluorescent protein (RFP), and thymidine kinase under the control of the same SBE promoter (SBE-lucRT mice). SBE-luc and SBE-lucRT mice can be used to study temporal, tissue-specific activation of Smad2/3-dependent signaling in living mice as well as for the identification of endogenous or synthetic modulators of this pathway
      13. URL :
        http://www.springerprotocols.com/Abstract/doi/10.1007/978-1-60327-321-3_16
      14. Call Number :
        140764
      15. Serial :
        5511