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      1. Author :
        Abate, Aida; Yang, Guang; Wong, Ronald J.; Schroder, Henning; Stevenson, David K.; Dennery, Phyllis A.
      2. Title :
      3. Type :
        Journal Article
      4. Year :
        2005
      5. Publication :
        Free Radical Biology and Medicine
      6. Products :
      7. Volume :
        39
      8. Issue :
        N/A
      9. Page Numbers :
        711
      10. Research Area :
        N/A
      11. Keywords :
        IVIS, Xenogen
      12. Abstract :
        Hemin is a strong inducer of heme oxygenase-1 (HO-1) expression in vitro and in vivo. Whereas moderate overexpression of HO-1 is protective against oxidative stress, uncontrolled levels of HO-1 can be detrimental. Therefore, we evaluated the effects of apigenin (APG), a flavonoid involved in a number of phosphorylation pathways and also known to inhibit inducible genes, such as iNOS and COX-2, on HO-1 expression. Incubation of mouse embryonic fibroblasts with APG (5-40 μM) decreased hemin-induced HO-1 protein and mRNA expression. APG also reduced the induction of HO-1 promoter activity, as assessed by bioluminescence imaging, in NIH3T3 cells transfected with the 15-kb HO-1 promoter fused with the reporter gene luciferase (HO-1-luc). Furthermore, through the use of specific inhibitors, APG's effect was found to be unrelated to its PKC, CK2, PI3K, p38, or ERK inhibitory activities. Quercetin (10-40 μM), also a flavonoid, also inhibited hemin-induced HO-1 expression. Additionally, in vivo studies using HO-1-luc transgenic mice showed that APG (50 mg/kg) decreased hemin-induced HO activity and HO-1 protein expression in the liver. These results suggest that hemin-induced HO-1 expression can be attenuated by flavonoids, such as APG.
      13. URL :
        http://www.sciencedirect.com/science/article/B6T38-4FK3NC1-1/2/d066ccaa2ec4dc79f60bc0a1599f4568
      14. Call Number :
        135712
      15. Serial :
        5444
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