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      1. Author :
        Lagerweij, Tonny; Dusoswa, Sophie A.; Negrean, Adrian; Hendrikx, Esther M. L.; de Vries, Helga E.; Kole, Jeroen; Garcia-Vallejo, Juan J.; Mansvelder, Huibert D.; Vandertop, W. Peter; Noske, David P.; Tannous, Bakhos A.; Musters, René J. P.; van Kooyk, Yvette; Wesseling, Pieter; Zhao, Xi Wen; Wurdinger, Thomas
      2. Title :
      3. Type :
        Journal Article
      4. Year :
        2017
      5. Publication :
        Angiogenesis
      6. Products :
      7. Volume :
        N/A
      8. Issue :
        N/A
      9. Page Numbers :
        N/A
      10. Research Area :
        N/A
      11. Keywords :
        Vasculature; Imaging; 3D; CLARITY; iDISCO; Multicellular network; IVIS; IVIS BLI in vivo
      12. Abstract :
        Three-dimensional visualization of the brain vasculature and its interactions with surrounding cells may shed light on diseases where aberrant microvascular organization is involved, including glioblastoma (GBM). Intravital confocal imaging allows 3D visualization of microvascular structures and migration of cells in the brain of mice, however, with limited imaging depth. To enable comprehensive analysis of GBM and the brain microenvironment, in-depth 3D imaging methods are needed. Here, we employed methods for optical tissue clearing prior to 3D microscopy to visualize the brain microvasculature and routes of invasion of GBM cells.
      13. URL :
        https://link.springer.com/content/pdf/10.1007%2Fs10456-017-9565-6.pdf
      14. Call Number :
        PKI @ catherine.lautenschlager @ 14083
      15. Serial :
        13972