Citation Details

Journal Article

Xen36, Xen 36, Staphylococcus aureus Xen36, IVIS, Animals; Arthroplasty; Biofilms/growth & development; Bone Wires/microbiology; Interleukin-1beta/*metabolism; Male; Mice; Mice, Congenic; Mice, Inbred C57BL; Myeloid Differentiation Factor 88/metabolism; Neutrophil Infiltration; Prosthesis-Related Infections/*immunology/metabolism; Staphylococcal Infections/*immunology/metabolism; Staphylococcus aureus; Toll-Like Receptor 2/*metabolism

MyD88 is an adapter molecule that is used by both IL-1R and TLR family members to initiate downstream signaling and promote immune responses. Given that IL-1beta is induced after Staphylococcus aureus infections and TLR2 is activated by S. aureus lipopeptides, we hypothesized that IL-1beta and TLR2 contribute to MyD88-dependent protective immune responses against post-arthroplasty S. aureus infections. To test this hypothesis, we used a mouse model of a post-arthroplasty S. aureus infection to compare the bacterial burden, biofilm formation and neutrophil recruitment in IL-1beta-deficient, TLR2-deficient and wild-type (wt) mice. By using in vivo bioluminescence imaging, we found that the bacterial burden in IL-1beta-deficient mice was 26-fold higher at 1 day after infection and remained 3- to 10-fold greater than wt mice through day 42. In contrast, the bacterial burden in TLR2-deficient mice did not differ from wt mice. In addition, implants harvested from IL-1beta-deficient mice had more biofilm formation and 14-fold higher adherent bacteria compared with those from wt mice. Finally, IL-1beta-deficient mice had approximately 50% decreased neutrophil recruitment to the infected postoperative joints than wt mice. Taken together, these findings suggest a mechanism by which IL-1beta induces neutrophil recruitment to help control the bacterial burden and the ensuing biofilm formation in a post-surgical joint.