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      1. Author :
        Sivankalyani, Velu; Sela, Noa; Feygenberg, Oleg; Zemach, Hanita; Maurer, Dalia; Alkan, Noam
      2. Title :
      3. Type :
        Journal Article
      4. Year :
        2016
      5. Publication :
        Frontiers in Plant Science
      6. Products :
      7. Volume :
        7
      8. Issue :
        1579
      9. Page Numbers :
        N/A
      10. Research Area :
        N/A
      11. Keywords :
        Transcriptome,; Mango fruit,; Fruit response,; Cold storage,; chilling injury,; Lenticel discoloration,; Lipid Peroxidation; IVIS; IVIS BLI in vitro
      12. Abstract :
        Cold storage is considered the most effective method for prolonging fresh produce storage. However, subtropical fruit is sensitive to cold. Symptoms of chilling injury in mango include red and black spots that start from discolored lenticels and develop into pitting. The response of ‘Keitt’ mango fruit to chilling stress was monitored by transcriptomic, physiological and microscopic analyses. Transcriptomic changes in the mango fruit peel were evaluated during optimal (12°C) and suboptimal (5°C) cold storage. Two days of chilling stress upregulated genes involved in the plant stress response, including those encoding transmembrane receptors, calcium-mediated signal transduction, NADPH oxidase, MAP kinases and WRKYs, which can lead to cell death. Indeed, cell death was observed around the discolored lenticels after 19 days of cold storage at 5°C. Localized cell death and cuticular opening in the lumen of discolored lenticels were correlated with increased general decay during shelf-life storage, possibly due to fungal penetration. We also observed increased phenolics accumulation around the discolored lenticels, which was correlated with the biosynthesis of phenylpropanoids that were probably transported from the resin ducts. Increased lipid peroxidation was observed during chilling injury by both the biochemical malondialdehyde method and a new non-destructive luminescent technology, correlated to upregulation of the α-linolenic acid oxidation pathway. Genes involved in sugar metabolism were also induced, possibly to maintain osmotic balance. This analysis provides an in-depth characterization of mango fruit response to chilling stress and could lead to the development of new tools, treatments and strategies to prolong cold storage of subtropical fruit.
      13. URL :
        http://journal.frontiersin.org/article/10.3389/fpls.2016.01579 http://journal-cdn.frontiersin.org/article/225485/files/pubmed-zip/versions/1/pdf
      14. Call Number :
        PKI @ user @ 12576
      15. Serial :
        19486
      1. Author :
        Yoshimura, Yuki; Nakamura, Kazuomi; Endo, Takeshi; Kajitani, Naoyo; Kazuki, Kanako; Kazuki, Yasuhiro; Kugoh, Hiroyuki; Oshimura, Mitsuo; Ohbayashi, Tetsuya
      2. Title :
        Mouse embryonic stem cells with a multi-integrase mouse artificial chromosome for transchromosomic mouse generation
      3. Type :
        Journal Article
      4. Year :
        2015
      5. Publication :
        Transgenic research
      6. Products :
      7. Volume :
        N/A
      8. Issue :
        N/A
      9. Page Numbers :
        N/A
      10. Research Area :
        N/A
      11. Keywords :
        Transchromosomic mice; Mouse artificial chromosome; Gene delivery; IVIS; IVIS BLI ex vivo
      12. Abstract :
        The mouse artificial chromosome (MAC) has several advantages as a gene delivery vector, including stable episomal maintenance of the exogenous genetic material and the ability to carry large and/or multiple gene inserts including their regulatory elements. Previously, a MAC containing multi-integration site (MI-MAC) was generated to facilitate transfer of multiple genes into desired cells. To generate transchromosomic (Tc) mice containing a MI-MAC with genes of interest, the desired genes were inserted into MI-MAC in CHO cells, and then the MI-MAC was transferred to mouse embryonic stem (mES) cells via microcell-mediated chromosome transfer (MMCT). However, the efficiency of MMCT from CHO to mES cells is very low (<10−6). In this study, we constructed mES cell lines containing a MI-MAC vector to directly insert a gene of interest into the MI-MAC in mES cells via a simple transfection method for Tc mouse generation. The recombination rate of the GFP gene at each attachment site (FRT, PhiC31attP, R4attP, TP901-1attP and Bxb1attP) on MI-MAC was greater than 50 % in MI-MAC mES cells. Chimeric mice with high coat colour chimerism were generated from the MI-MAC mES cell lines and germline transmission from the chimera was observed. As an example for the generation of Tc mice with a desired gene by the MI-MAC mES approach, a Tc mouse strain ubiquitously expressing Emerald luciferase was efficiently established. Thus, the findings suggest that this new Tc strategy employing mES cells and a MI-MAC vector is efficient and useful for animal transgenesis.
      13. URL :
        N/A
      14. Call Number :
        PKI @ user @ 9633
      15. Serial :
        20220
      1. Author :
        Xu, Tingting; Marr, Enolia; Lam, Haylie; Ripp, Steven; Sayler, Gary; Close, Dan
      2. Title :
        Real-time toxicity and metabolic activity tracking of human cells exposed to Escherichia coli O157: H7 in a mixed consortia
      3. Type :
        Journal Article
      4. Year :
        2015
      5. Publication :
        Ecotoxicology
      6. Products :
      7. Volume :
        N/A
      8. Issue :
        N/A
      9. Page Numbers :
        N/A
      10. Research Area :
        N/A
      11. Keywords :
        Toxicology; Cell culture; E. coli O157:H7; Bioluminescence; Optical imaging; IVIS; IVIS BLI
      12. Abstract :
        Escherichia coli O157:H7 is a significant human pathogen that is continually responsible for sickness, and even death, on a worldwide scale. While the pathology of E. coli O157:H7 infection has been well studied, the effect of it’s multiple resulting cytotoxic mechanisms on host metabolic activity has not been well characterized. To develop a more thorough understanding of these effects, several bioluminescence assays were evaluated for their ability to track both toxicity and host metabolic activity levels in real-time. The use of continuously autobioluminescent human cells was determined to be the most favorable method for tracking these metrics, as its self-sufficient autobioluminescent phenotype was unaffected by the presence of the infecting bacteria and its signal could be measured without cellular destruction. Using this approach, it was determined that infection with as few as 10 CFU of E. coli O157:H7 could elicit cytotoxic effects. Regardless of the initial infective dose, an impact on metabolic expression was not observed until bacterial populations reached levels between 5 × 105 and 1 × 106 (R2 = 0.933), indicating that a critical bacterial infection level must be reached prior to the onset of cytotoxic effects. Supporting this hypothesis, it was found that cells displaying infection-mediated metabolic activity reductions could recover to wild type metabolic activity levels if the infecting bacteria were removed prior to cell death. These results indicate that rapid treatment of E. coli O157:H7 infection could serve to limit host metabolic impact and reduce overall host cell death.
      13. URL :
        N/A
      14. Call Number :
        PKI @ user @ 10178
      15. Serial :
        20248
      1. Author :
        George, Adia G.; Yang, Guang; Dennery, Phyllis A.
      2. Title :
      3. Type :
        Journal Article
      4. Year :
        2004
      5. Publication :
        Pediatric Research
      6. Products :
      7. Volume :
        55
      8. Issue :
        N/A
      9. Page Numbers :
        291
      10. Research Area :
        N/A
      11. Keywords :
        Toxicology, Blood and Lymphatics (Transport and Circulation), Molecular Genetics (Biochemistry and Molecular Biophysics), Cell, Biology, Digestive System (Ingestion and Assimilation), 00520, General biology – Symposia, transactions and proceedings, 02502, Cytology – General, 02506, Cytology – Animal, 02508, Cytology -, Human, 03502, Genetics – General, 03506, Genetics – Animal, 03508,, Genetics – Human, 10062, Biochemistry studies – Nucleic acids, purines, and pyrimidines, 10064, Biochemistry studies – Proteins, peptides and, amino acids, 10065, Biochemistry studies – Porphyrins and bile, pigments, 14004, Digestive system – Physiology and biochemistry, 15002, Blood – Blood and lymph studies, 15004, Blood – Blood cell, studies, 22501, Toxicology – General and methods, 25000, Pediatrics, Primates, Mammalia, Vertebrata, Chordata, Animalia, Animals,, Chordates, Humans, Mammals, Primates, Vertebrates, Hominidae [86215], [human, (newborn, adult)]/Rodentia, Mammalia, Vertebrata, Chordata,, Animalia, Animals, Chordates, Mammals, Nonhuman Vertebrates, Nonhuman, Mammals, Rodents, Vertebrates, Muridae [86375], [mouse, (newborn, strain-C57BL/6)], DNA/hemoglobin/serum proteins/zinc protoporphyrin, 15442-64-5, (toxin,, cytotoxin), light microscopy, laboratory techniques, imaging and microscopy, techniques/polymerase chain reaction, laboratory techniques, genetic, techniques/immunoprecipitation, laboratory techniques, immunologic, techniques/centrifugation, laboratory techniques/DNA fragmentation, laboratory techniques, genetic techniques/non-denaturing gel, electrophoresis, electrophoretic techniques, laboratory, techniques/IVIS imaging system, Xenogen Alameda, California, laboratory equipment, liver, digestive system/serum, blood and lymphatics/red blood cell, blood and lymphatics/blood, blood and lymphatics, fetal cord IVIS, Xenogen
      12. Abstract :
        .
      13. URL :
        ://BIOSIS:PREV200600124157"">://BIOSIS:PREV200600124157
      14. Call Number :
        138019
      15. Serial :
        5498
      1. Author :
        Anand, Sanjay; Bullock, Taylor; Maytin, Edward V.
      2. Title :
      3. Type :
        Journal Article
      4. Year :
        2017
      5. Publication :
        N/A
      6. Products :
      7. Volume :
        10047
      8. Issue :
        N/A
      9. Page Numbers :
        N/A
      10. Research Area :
        N/A
      11. Keywords :
        Toxicity ;; Breast ;; Breast cancer ;; Ionizing radiation ;; Mouth ;; Photodynamic therapy ;; Prostate ;; Radiation ;; Skin ;; Tissues; IVIS; IVIS BLI in vivo
      12. Abstract :
        Cutaneous metastasis (CM) occurs in 20% of patients with breast carcinoma (BCA), and is extremely difficult to treat. These CM are relatively resistant to chemotherapy, generally responding only to ionizing radiation (IR). Multiple rounds of IR, however, lead to debilitating fibrosis and radiation dermatitis. An alternative to IR is needed for better management of BCA/CM. In our laboratory, we have developed differentiation-enhanced combination PDT (cPDT), a concept in which a pro-differentiating agent (methotrexate; vitamin D; or 5-fluorouracil, 5FU) is used as a neoadjuvant prior to PDT. After using these neoadjuvants, levels of protoporphyrin IX (PpIX) were elevated in animal tumor models of skin, prostate, and BCA, leading to better PDT efficacy. However, all the agents have toxicity issues. Here, we use a nontoxic 5FU precursor called Capecitabine (CPBN) for cPDT. CBPN is a standard chemotherapeutic for metastatic BCA, and is metabolized to 5FU specifically within tumor tissue. Murine (4T1) and human (MCF-7) BCA cell lines were injected into breast fat pads of nude mice. After tumor nodules appeared, CPBN (400-600 mg/kg/day) was administered by oral gavage for five days followed by intraperitoneal ALA administration on day 6. Mice were sacrificed and tumors harvested. CPBN pretreatment led to a 4-fold elevation of PpIX levels in tumors, relative to vehicle control. Not only did PpIX levels increase, but also PpIX distribution became more homogeneous after CPBN pretreatment. In summary, the use of non-toxic CPBN as a neoadjuvant prior to PDT is a combination approach with significant potential for translation into the clinic.
      13. URL :
        N/A
      14. Call Number :
        PKI @ catherine.lautenschlager @ 13334
      15. Serial :
        14216
      1. Author :
        Rothbard, Jonathan B.; Jones, Lisa R.
      2. Title :
      3. Type :
        Journal Article
      4. Year :
        2011
      5. Publication :
        Methods in Molecular Biology
      6. Products :
      7. Volume :
        683
      8. Issue :
        N/A
      9. Page Numbers :
        N/A
      10. Research Area :
        N/A
      11. Keywords :
        Topical drug delivery, Drug conjugates, Luciferin, Bioluminescence IVIS, Xenogen
      12. Abstract :
        A major challenge confronting the further advancement of using molecular transporters conjugated to small molecular weight therapeutics in the clinic is the development of linkers that would allow for the controllable release of a free drug/probe only after cell entry. Development of assays that would allow for the rapid real-time quantification of transporter conjugate uptake and cargo release in cells and animals would greatly help in their development. In this chapter, we describe a imaging method that quantitatively measures transporter conjugate uptake and cargo release in real-time in both cell culture and animal models.
      13. URL :
        http://www.springerprotocols.com/Abstract/doi/10.1007/978-1-60761-919-2_35
      14. Call Number :
        142660
      15. Serial :
        7418
      1. Author :
        Wang, Kan; Wang, Qun; Luo, Qingming; Yang, Xiaoquan
      2. Title :
      3. Type :
        Journal Article
      4. Year :
        2015
      5. Publication :
        Optics Express
      6. Products :
      7. Volume :
        23
      8. Issue :
        10
      9. Page Numbers :
        N/A
      10. Research Area :
        N/A
      11. Keywords :
        Tomography; Fluorescence; FMT
      12. Abstract :
        Fluorescence molecular tomography (FMT), an in vivo noninvasive imaging technology, can provide localization and quantification information for deep fluorophores. Light at wavelengths in the near-infrared (NIR-I) window from 650 nm to 950 nm has conventionally been chosen for FMT. In this study, we introduced longer NIR wavelengths within the 1100 nm to 1400 nm range, known as the “second NIR spectral window” (NIR-II). A singular-value analysis method was used to demonstrate the utility and advantages of using the NIR-II for FMT, and experiments showed an improvement in the spatial resolution in phantom studies.
      13. URL :
        http://www.opticsexpress.org/abstract.cfm?URI=oe-23-10-12669
      14. Call Number :
        PKI @ catherine.lautenschlager @ 9567
      15. Serial :
        13471
      1. Author :
        Zou, Wei; Wang, Jiajun; Hu, Danfeng; Wang, Wenxia
      2. Title :
        A reconstruction approach in wavelet domain for fluorescent molecular tomography via rotated sources illumination
      3. Type :
        Journal Article
      4. Year :
        2015
      5. Publication :
        BioMedical Engineering OnLine
      6. Products :
      7. Volume :
        14
      8. Issue :
        1
      9. Page Numbers :
        N/A
      10. Research Area :
        N/A
      11. Keywords :
        Tomography,; Wavelet,; Reconstruction; FMT
      12. Abstract :
        Background: Fluorescent molecular tomography (FMT) aims at reconstructing the spatial map of optical and fluorescence parameters from fluence measurements. Basically, solving large-scale matrix equations is computationally expensive for image reconstruction of FMT. Despite the reconstruction quality can be improved with more sources, it may result in higher computational costs for reconstruction. This article presents a novel method in the wavelet domain with rotated sources illumination. Methods: We use the finite element method for the computation of the forward model. The global inverse problem is solved based on wavelet in conjunction with principal component analysis. The iterative reconstruction is implemented with sources rotated in a certain angle. The original excitation light sources are used to reconstruct the image in the first iteration. Then, upon the sources are rotated by a certain angle, they are employed for the next iteration of reconstruction. Results: Simulation results demonstrate that our method can considerably reduce the time taken for the computation of inverse problem in FMT. Furthermore, the approach proposed is also shown to largely outperform the traditional method in terms of the precision of inverse solutions. Conclusions: Our method has the capability to locate the inclusions. The proposed method can significantly speed up the reconstruction process with the high reconstruction quality.
      13. URL :
        N/A
      14. Call Number :
        PKI @ catherine.lautenschlager @ 10168
      15. Serial :
        13461
      1. Author :
        Zhou, Yuan; Guang, Huizhi; Pu, Huangsheng; Zhang, Jiulou; Luo, Jianwen
      2. Title :
      3. Type :
        Journal Article
      4. Year :
        2016
      5. Publication :
        Applied Optics
      6. Products :
      7. Volume :
        55
      8. Issue :
        18
      9. Page Numbers :
        N/A
      10. Research Area :
        N/A
      11. Keywords :
        Tomographic image processing; Tomography; Fluorescence; FMT
      12. Abstract :
        Fluorescence molecular tomography (FMT) can visualize biological activities at cellular and molecular levels in vivo, and has been extensively used in drug delivery and tumor detection research of small animals. The ill-posedness of the FMT inverse problem makes it difficult to reconstruct and unmix multiple adjacent fluorescent targets that have different functional features but are labeled with the same fluorochrome. A method based on independent component analysis for multispectral excited FMT was proposed in our previous study. It showed that double fluorescent targets with certain edge-to-edge distance (EED) could be unmixed by the method. In this study, the situation is promoted to unmix multiple adjacent fluorescent targets (i.e., more than two fluorescent targets and EED=0). Phantom experiments on the resolving ability of the proposed algorithm demonstrate that the algorithm performs well in unmixing multiple adjacent fluorescent targets in both lateral and axial directions. And also, we recovered the locational information of each independent fluorescent target and described the variable trends of the corresponding fluorescent targets under the excitation spectrum. This method is capable of unmixing multiple fluorescent targets with small EED but labeled with the same fluorochrome, and may be used in imaging of nonspecific probe targeting and metabolism of drugs.
      13. URL :
        http://ao.osa.org/abstract.cfm?URI=ao-55-18-4843
      14. Call Number :
        PKI @ catherine.lautenschlager @ 11811
      15. Serial :
        13411
      1. Author :
        Schwandt, T.; Juengerkes, F.; Schumak, B.; Holzmann, B.; Layland, L.; Limmer, A.
      2. Title :
        Long-term effects of sepsis: The influence of bacteremia and bacterial translocation on systemic adaptive immune responses
      3. Type :
        Journal Article
      4. Year :
        2010
      5. Publication :
        Inflammation Research
      6. Products :
      7. Volume :
        59
      8. Issue :
        N/A
      9. Page Numbers :
        N/A
      10. Research Area :
        N/A
      11. Keywords :
        toll like receptor 4, interferon, tumor necrosis factor alpha, interleukin 10, cytokine, noscapine, antigen, Europe, bacteremia, adaptive immunity, sepsis, bacterial translocation, inflammation, surgical infection, injury, mouse, Escherichia coli, bacterium, liver, spleen, infection, lung, intestine, septic shock, abdominal surgery, cytotoxic T lymphocyte, Adenovirus, injection, portal vein, Kupffer cell, filter, immunity, risk, patient, opportunistic infection, model, ascending colon, stent, peritonitis, imaging, microbiology, Listeria monocytogenes IVIS, Xenogen
      12. Abstract :
        Objective: Bacterial translocation is a possible risk of abdominal surgery and may cause life-threatening consequences such as organ failure and septic shock. Patients surviving septic shock often suffer from opportunistic infections as well as defects in adaptive immunity. Methods: Here we investigated the influence of bacteremia and bac-terial translocation on systemic adaptive immune responses using murine models. To mimic abdominal surgery, mice were subjected to intestinal manipulation (IM). To study septic conditions, mice underwent colon ascendens stent peritonitis (CASP) or received E. coli intravenously or intraportally. We monitored the distribution of gut-derived bacteria by in vivo imaging (Xenogen) and additional microbiological assays and determined antigen-specific cytotoxic T lymphocyte (CTL) responses towards subsequent infection with recombinant adenoviruses (rAV) or Listeria monocytogenes. Results: We identified a strong correlation between the presence of bacteria in the spleen and a suppression of the CTL response, which was observed in mice that underwent CASP or were injected i.v. with E. coli. In contrast, the CTL response was not impaired in mice that were subjected to IM, or received E. coli by injection into the hepatic portal vein. Here, bacteria were detected in lung and liver but not in the spleens of mice. Depletion experiments implied that Kupffer cells as well as soluble mediators such as tumor necrosis factor alpha played an important role in trapping and clearance of translocated bacteria in liver and lung. Importantly, we figured out, that mice deficient in TLR-4 or in both MyD88 and TRIF showed no impaired CTL response after systemic E. coli infection. Astonishingly, IL-10-known as an immunosuppres-sive cytokine-did not play any role in E. coli-induced suppression of CTL response but rather type I IFN were shown to represent essential effector molecules as in mice deficient in IFNAR or IRF3/7 generated normal CTL responses after E. coli infection. Conclusion: We suggest that liver and lung fulfill a filter function to prevent systemic distribution of gut-derived bacteria. Failure of or bypassing these barriers might enable bacteria to access the spleen and thus cause systemic suppression of adaptive immunity, whereas the induction of local immunity was not affected. Suppression of CTL responses was strongly dependent on TLR-4 and mediated by downstream signaling of TRIF and MyD88 such as type I interferons.
      13. URL :
        N/A
      14. Call Number :
        142912
      15. Serial :
        6783
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